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KMID : 0385219950050020059
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Korean Journal of Gerontology
1995 Volume.5 No. 2 p.59 ~ p.62
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Inhibition of Superoxide Dismutase in Human Amnion, Chorion and Placenta by Cyanide and Azide
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Kim Seok-Bae
Han Ki-Jung
Baek Kwang-Jin
Kwon Nyoun-Soo
Chi Kyong-Chon
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Abstract
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Distribution of cytosolic and mitochondrial superoxide dismutase in human amnion, chorion and placenta and their inhibition by cysnide and azide were investigated. Activities of superoxide dismutase were 118.43±3.29 U/g of chorion, 49.09±5. 57 U/g of amnion and 31.55±3.17 U/g of placenta. In amnion, predominsnt activity (93%) was found in cytosolic fraction complete inhibition of the activities of cytosolic superoxide dismutase in amnion, chorion and placenta were achieved by 5 mM, 10 mM and 6 mM cyanide, respectively. Mitochondrial superoxide dismutase from amnion and placenta were completely inactivated by 8 mM and 5 mM cyanide, respectively. However, only 40% of chorionic mitochondrial superoxide dismutase was inactivated by 10 mM cyanide. Inhibitory effect of azide was more remarkable to the cytosolic enzyme than to the mitochondrial enzyme.
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KEYWORD
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cyanide, azide, amnion, chorion, placenta, superoxide dismutase
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